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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Immunol. 2011 Dec 12;49(4):628–639. doi: 10.1016/j.molimm.2011.11.002

Figure 3. Expression of tapasin Δ334-342 partially restores surface expression of Kd molecules, whereas expression of soluble tapasin, alone or with tapasin Δ334-342, does not improve Kd levels at the plasma membrane.

Figure 3

(A) Cell surface expression of Kd on the indicated cell lines was determined by flow cytometry using the 64-3-7 and 34-1-2 mAbs. (B) Cell surface expression of Kd molecules on MF-Kd-SOL and MF-Kd-SOL-ΔTsn cell lines, as determined by the flow cytometric analysis performed in (A), but here shown in expanded detail. (C) The ratio of open to folded Kd at the cell surface was calculated by dividing the mean fluorescence units obtained with the 64-3-7 mAb by the mean fluorescence units obtained with the 34-1-2 mAb. Data are representative of 5 independent flow cytometric analyses.