Table 1.
The ES and LS characteristics of proteins under consideration. The position of mutations is given in the second column, followed by the value of D (distance), which is a measure of the accordance with the adopted model of ES intermediate. The D average expresses the mean distance between the projected and observed values of parameters that describe the structure of ES intermediate. The proteins with D average values below 1.0 are considered consistent with the ES model. The protein of the relation O/T < O/R is interpreted as accordant with LS model O/T denotes the Kullback-Leibler entropy calculated for the observed (O) distribution of hydrophobicity density and theoretical one (T) treated as the target distribution in comparison with the O/R expressing the distance between observed one and random (R) treated as the target distribution. Chains A were taken for analysis in NMR technique determining the protein structure. The values given in bold denote the case of accordance with appropriate model
| PDB - ID | Mutation | D average | O/T | O/L |
|---|---|---|---|---|
| 1AME | P64A, P65A | 1.214 | 0.058 | 0.066 |
| 2AME | P64A, P65A, N14Q | 1.174 | 0.062 | 0.072 |
| 3AME | P64A, P65A, Q9T, Q44T | 1.102 | 0.055 | 0.060 |
| 4AME | P64A, P65A, T18A | 1.164 | 0.061 | 0.066 |
| 6AME | P64A, P65A, M21A | 1.202 | 0.059 | 0.069 |
| 7AME | P64A, P65A, T15A | 1.354 | 0.058 | 0.067 |
| 8AME | P64A, P65A, N14S, A16H | 1.251 | 0.059 | 0.066 |
| 9AME | P64A, P65A, S42G | 1.403 | 0.060 | 0.068 |
| 1MSI | P64A, P65A | 1.190 | 0.065 | 0.066 |
| 2MSI | A16M | 1.251 | 0.080 | 0.062 |
| 3MSI | A16H | 1.334 | 0.077 | 0.059 |
| 4MSI | A16T | 1.396 | 0.068 | 0.061 |
| 5MSI | A16C | 1.318 | 0.071 | 0.061 |
| 6MSI | A16R | 1.303 | 0.067 | 0.057 |
| 7MSI | A16Y | 1.294 | 0.066 | 0.060 |
| 8MSI | P64A, P65A, N14S, Q44T | 1.192 | 0.056 | 0.062 |
| 9MSI | P64A, P65A, T18N | 1.238 | 0.054 | 0.062 |
| 1MSJ | P64A, P65A, T15V | 1.237 | 0.060 | 0.068 |
| 2MSJ | P64A, P65A, N46S | 1.163 | 0.058 | 0.064 |
| 1JAB | P64A, P65A, T18A | 1.153 | 0.058 | 0.067 |
| 1JIA | P64A, P65A, K61I | 1.213 | 0.060 | 0.064 |
| 1B7I | P64A, P65A, K61R | 1.275 | 0.055 | 0.063 |
| 1B7J | P64A, P65A, V20A | 1.273 | 0.057 | 0.067 |
| 1B7K | P64A, P65A, R47H | 1.315 | 0.055 | 0.066 |
| 1KDE | INS(M0) P64A, P65A, INS(K66, D67, E68, L69) | 1.113 | 0.061 | 0.091 |
| 1KDF | INS(M0) P64A, P65A, INS(K66, D67, E68, L69) | 0.989 | 0.059 | 0.076 |
| 2SPG | T15S | 1.381 | 0.060 | 0.067 |
| 1J5B | T(2,13,24,35)V A(7,29)K, A(11,33)E | 0.192 | 0.349 | 0.072 |