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. Author manuscript; available in PMC: 2012 Jan 12.
Published in final edited form as: Eur Cell Mater. 2011 Jan 12;21:31–45. doi: 10.22203/ecm.v021a03

Fig. 1. Aggrecanolysis in response to cytokine stimulation.

Fig. 1

Bovine articular cartilage explants were cultured in the presence of IL-1β, TNFα and OSM, alone or in combination for two days, when culture media were analyzed for GAG content by the DMMB assay (a), generation of aggrecan G3 (b) and G1 (c) -containing fragments, and LP release (d) by immunoblotting. The different cytokine treatments are indicated below the figures. The migration positions of known aggrecanase-generated products by cleavage in the IGD (G1) (Panel c) and CS-2 (Panel b) region (fragments 2–6) of aggrecan, and the position of LP (Panel d) are indicated on the right. Migration positions of molecular weight markers (kDa) are indicated on the left. The ~26 kDa band represents biotinylated SBTI used as a loading control. * indicates p<0.02 for GAG release as determined by unpaired t-test compared to control or as indicated by the brackets.