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. 2011 Dec 20;2012:974067. doi: 10.1155/2012/974067

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Copyright © 2012 Juliana Assis Silva Gomes et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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B cell (CD19⁺) activation status in PBMCs from individuals infected or not by zoonotic Vaccinia virus. Peripheral blood mononuclear cells (PBMCs) from patients infected or not with zoonotic Vaccinia virus were cultured in the presence of UV-inactivated virus or mock-treated (medium). After 72 hours of stimulus, cells were fixed, counted, and the following parameters were evaluated by flow cytometry using specific mouse anti-human antibodies: (a) percentage of B lymphocytes (CD19⁺) expressing CD86 surface activation marker; (b) percentage of B lymphocytes (CD19⁺) expressing CD80 surface activation marker. Statistical significance (P values), based on the median values of each group, is presented on the graphs.