Figure 8.

Aspects of the immune responses in mice infected or not with zoonotic Vaccinia virus. Peripheral blood mononuclear cells (PBMCs) from mice either infected with a zoonotic Vaccinia virus isolate or noninfected were cultured for 72 hours in the presence of UV-inactivated VACV. After incubation, cells were labeled with specific antibodies and analyzed by flow cytometry. (a) Percentage of total monocytes (CD14⁺); (b) percentage of CD4⁺ T lymphocytes expressing the CD25 surface activation marker; (c) percentage of CD8⁺ T lymphocytes. Bars represent the mean results from 10 animals. Error bars and P values are indicated.