| Step | T (°C) | Start volt (V) | End volt (V) | Duration (h) | Total (Vh) |
|---|---|---|---|---|---|
| IEF protocol (7 cm) | |||||
| 1 | 20 | 0 | 250 | 0.5 | |
| 2 | 20 | 250 | 1500 | 1 | |
| 3 | 20 | 1500 | 3000 | 1 | |
| 4 | 20 | 3000 | 8000 | 3 | 11,000 |
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