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Growth of full-length WNV infectious clone (–––) and WNV mutants (- - -) in C6/36, DF-1 and Vero cell cultures. Single mutants are WNV A1712G and WNV G6687T. WNV E/NS4 contains the mutations A1712G and G6687T. The m.o.i. for all growth curves was 0.01 p.f.u. per cell. Results are presented as the means ± sd of duplicate assays.
