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. 2011 Nov 22;153(1):42–55. doi: 10.1210/en.2011-1308

Fig. 5.

Fig. 5.

Relative gene expression was determined by real-time PCR for (A) Dnmt1, (B) Dnmt3a, (C) Dnmt3b, (D) Mecp2, (E) Mbd1, (F) Mbd3, (G) Mbd2, and (H) Mbd4. RNA was isolated from the dorsal prostate of animals treated with HEB (blue bar), LEB (red bar), BPA (yellow bar), or oil (gray bar) at d 10, 90, or 200 (±T+E2). Relative expression ratio (RER) was calculated by normalizing transcript levels of a gene to that of Rpl19 transcript level in the same sample. The average RER in samples from d 10 oil-treated prostates was arbitrarily assigned a value of 1.0 for each gene analyzed. Levels of transcripts in all other treatment groups at various life stages were normalized to the oil-treated controls. Statistical significant differences are indicated (*, P < 0.05, when comparing values in EB/BPA-treatment groups with oil-treated controls; #, P < 0.05, when values in older control animals were compared with values in d 10 controls).