Fig. 6.

Celecoxib treatment significantly suppressed Rankl, Pparg2, and Cox-2 expression in the femur of Alox5^−/− mice fed a HFD. A–D, Alox5^−/− mice were fed a control diet (10% fat by kcal) or a HFD (45% fat by kcal) supplemented with 1000 ppm celecoxib from 3 wk of age for 8 wk. RNA was collected from the femur at 11 wk of age, and the expression of Rankl (A), Opg (B), Pparg2 (C), and Cox-2 (D) was analyzed by real-time RT-PCR (n ≥ 5). Values are expressed as the mean ± sem. *, P < 0.02; **, P < 0.05.