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. 2011 Jul-Dec;6(2):87–92.

Fig. 1.

Fig. 1

Western blot analysis by ECL method of extracts from E. coli strain DE3 containing recombinant plasmid pET15b/reteplase (rpET-BL21) using mouse anti His antibody. Lane: L) Protein marker, Lane:1 extract from E. coli strain DE3 bacteria containing plasmid pET15b, Lane:2) extract from E. coli strain DE3 bacteria, Lane:3) pET-BL21 induction with 1 mM IPTG, Lane:4) extracts from E. coli strain DE3 bacteria containing recombinant plasmid (rpET15b). A band with molecular mass of approximately 39 kDa was detected. Lane:5) extract from E. coli strain DE3 bacteria