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Induction of the expression of reteplase with 1 mM IPTG for 2 h using different shaking speeds. Proteins in the whole cell lysates were separated using SDS-PAGE Lanes:1 and 2): rpET-BL21 induced at 100 rpm, Lanes:3 and 4): rpET-BL21 induced at 170 rpm, Lanes:5 and 6): rpET-BL21 induced at 190 rpm. Lane:7) pET-BL21 induced at 100 rpm, Lane:8) pETBL21 induced at 170 rpm, Lane:9) pET-BL21 induced at 190 rpm and Lane:10) protein marker. Arrow shows the 39 kDa reteplase band.
