Estrogen receptor α and β expression in ovarian tumors.
Immunohistochemistry. Tumors were stained for estrogen receptor α (A) and β (B) expression using a mouse antibody. Representative tumor samples from each treatment group were stained and quantification was performed using an Automated Cellular Imaging System. A region score was calculated as the ratio of positively stained nuclei to all nuclei. Ten representative regions were analyzed per slide. Original magnification, 200x and 400x. Scale bar = 50 μm. Columns, mean region score; bars, SD; *, P<0.5. Groups: Ctrl= vehicle control, E2= 17β-estradiol, B= bazedoxifene