Fig. 7.

Effect of RTV on the formation of semicarbazide conjugated LPV adducts. Duplicate incubations were conducted in PBS (pH 7.4) containing LPV (30 μM), semicarbazide (2.5 mM), NADPH (1.0 mM), and HLM (1.0 mg/ml). RTV (2.0 μM) was used in the inhibitory test. All samples were analyzed by UPLC-TOFMS. The abundance of each adduct was relatively quantified using peak areas and presented as mean. ND, not detected.