Fig. 4.

The effect of desipramine on platinum compound cellular accumulation. HCT116 cells were treated with equimolar concentrations (20 μmol/L) of c-DDP and oxaliplatin for 16 h and of BBR3464 for 8 h, with the indicated concentrations of desipramine. Cellular platinum content was measured by inductively coupled plasma optical emission spectroscopy as described in “Materials and methods.” Each point represents the average (±SEM) of three independent experiments. Asterisks p < 0.05 as compared with drug alone as determined by the t test