Table 2.
# Primary Spheres | # Secondary Spheres* | # Differentiated Spheres** | |
---|---|---|---|
Wild type pericytes | 10 | 29 ± 10 | 15 ± 11 |
Wild type pericytes + ECsa | 25 | 49 ± 22*** | 35 ± 21*** |
IMPs | 31 | 56 ± 19 | 25 ± 11 |
IMPs + ECs | 45 | 71 ± 30*** | 42 ± 25*** |
Primary spheres were disrupted after two weeks (mean ± SD).
Mean number of differentiated spheres in secondary sphere preparation after two weeks (n = 2 experiments, > 10 primary spheres disrupted per experiment).
Significantly different from wild type pericyte and IMP cultures (P < 0.01).
5 × 104 ECs were added to 104 pericytes, the density of cells resuspended in stem cell medium was equal.