Figure 1.

Normal and FECD immortalized cell lines (HCECi and FECDi, respectively) were treated with increasing concentrations of tBHP and stained with Annexin V (Ann) and PI. Early apoptosis (Ann+/PI−) was measured using quantitative flow cytometry. The 4-hour treatment (A) resulted in similar levels of apoptotic cells in both HCECi and FECDi. However, the 14-hour treatment (B) resulted in significantly higher apoptosis in FECDi (*P < 0.05). (C) A representative image of flow cytometric analysis of HCECi and FECDi treated with 500 μM tBHP for 14 hours is illustrated.