Figure 6.

Substratum compliance and Lat-B treatment affects HTM cell migration. Before Lat-B treatment, HTM cells on 25 and 90 kPa surfaces migrated at an average rate of 1.45 and 1.72 μm/minute, respectively. While not statistically significant, both migration rates were faster than the 1.08 μm/minute measured on TCP surfaces. Migration rates on all three surfaces were significantly faster than the 0.37 μm/minute migration rate of HTM cells on 4 kPa surfaces before Lat-B treatment. HTM cell migration decreased significantly during Lat-B treatments and did not recover on any of the substrates tested including the 4 kPa surfaces. All data are reported as mean ± SEM (***P < 0.001).