Expression of the CYP3A4 mRNA transcripts determined by RNA sequencing (A) (n = 1) and RT-qPCR (B) (n = 3) in differentiated HepaRG cells after drug treatments. The differentiated HepaRG cells were treated with DMSO solvent control (0.1%), RIF (10 μM), or PB (750 μM) for 24 h. In the RT-qPCR experiment, the average expression level treated with DMSO is set as 1. Fold changes of expression levels of the transcripts after drug treatment are compared with the values in DMSO. Black column, mean; bar, S.D. of biological triplicate samples.