Fig. 7.
mRNA stability of the CYP3A4 transcripts. Levels of the CYP3A4 mRNA transcripts were determined by RT-qPCR in differentiated HepaRG cells at 0, 2, 4, and 6 h after the treatment of a transcription inhibitor, actinomycin D (2 μM) (n = 3). Fold changes of the mRNA transcript levels at 2, 4, and 6 h were compared with the levels at 0 h (considered as 1). Fold changes (ln) were plotted with the different time points (0, 2, 4, and 6 h). Half-life of mRNA was calculated at t1/2 = 0.693/(lnC1-lnC2)/t, with t = time interval between C1 and C2; C1 = amount of mRNA at t = 0, and C2 = amount of mRNA at t = 2, 4, or 6 h, respectively.