Skip to main content
. 2012 Jan;81(1):86–96. doi: 10.1124/mol.111.074393

Fig. 8.

Fig. 8.

Luciferase activity from plasmid constructs containing different CYP3A4 3′-UTR sequences. The plasmids were constructed by inserting different CYP3A4 3′-UTR sequences into the downstream region of a firefly luciferase gene in a pMir-Target Vector. Luciferase activity was used for detecting protein synthesis with the shorter CYP3A4 3′-UTR, canonical + shorter 3′-UTR, and canonical 3′-UTR alone (n = 5). Red fluorescence produced by an RFP was used for normalizing the transfection efficiency. In the pMir-Target vector: CMV, cytomegalovirus promoter; PolyA (downstream of Luciferase), human growth hormone poly(A) sequence (586 bp); SV40, simian vacuolating virus 40 promoter; Neo/Kanr, neomycin/kanamycin resistant; IRES, internal ribosome entering sequence; Luciferase, firefly luciferase; PolyA (downstream of RFP), HSV thymidine kinase poly(A) sequence (419 bp); F1 Ori, F1 phage origin of replication.