. 2012 Jan;81(1):86–96. doi: 10.1124/mol.111.074393

TABLE 1.

The primer sequences

Accession numbers: CYP3A4, NM_017460.3; GAPDH, NM_002046.3; ACTB, NM_001101.3.

Gene and Method	Amplification	Direction	Sequence (5′ to 3′)	PCR Product
				bp
CYP3A4
cDNA cloning	5′-UTR		`CACATAGCCCAGCAAAGAGCAACAC`
	3′-UTR		`ATTTATGCAGTCCATTGGATGAAGCC`
	Nested PCR		`CGGggtaccTGAAAGGAAGACTCAGAGGAGAGAG`
	Nested PCR		`GCtctagaGGTCTCTGGTGTTCTCAGGCACAG`
End-point PCR	From exon 5 to intron 6	F	`TTGCTGTCTCCAACCTTCACCAGT`	255
End-point PCR	From exon 5 to intron 6	R	`GTTGCATTACCACAGCCCTCCTTT`
3′RACE-PCR	cDNA synthesis		`GCGAGCACAGAATTAATACGACTCACTATAGGT 12VN`
	Outer PCR	F	`CTGCATTGGCATGAGGTTTGCTCT`
	Outer PCR	R	`GCGAGCACAGAATTAATACGACT`
	Inner PCR	F	`TTGAGTCAAGGGATGGCACCGTAA`
	Inner PCR	R	`CGCGGATCCGAATTAATACGACTCACTATAGG`
	Sequencing		`GTGACCAAATCAGTGTGAGGAGGT`
RT-PCR	Canonical 3′-UTR	F	`AATCCTAGCAGTTTGGGAGGCTGA`	142
RT-PCR	Canonical 3′-UTR	R	`TGAGATTACAGGCGAGTCCACCAT`
	Canonical and Short 3′-UTR	F	`TTGAGTCAAGGGATGGCACCGTAA`	156
	Canonical and Short 3′-UTR	R	`ATGCAGTCCATTGGATGAAGCCCA`
	Intron-6 retention	F	`GAGCTGATATTCCTGCTGTTGGGT`	85
	Intron-6 retention	R	`TCTTGGGAGACCCATTGAAGTTGC`
GAPDH
RT-PCR	From exon 4 to exon 6	F	`AATCCCATCACCATCTTCCAGGAG`	199
RT-PCR	From exon 4 to exon 6	R	`CATGGTTCACACCCATGACGAACA`
ACTB
RT-PCR	From exon 3 to exon 4	F	`ACCAACTGGGACGACATGGAGAAA`	192
RT-PCR	From exon 3 to exon 4	R	`TAGCACAGCCTGGATAGCAACGTA`

F, forward; R, reverse; 3′RACE-PCR, rapid amplification of cDNA 3′ ends-PCR