Fig. 2.

Expression of NBS1 and VHL genes loaded into the HAC in CHO cells. (A) FISH analysis of the alphoid^tetO-HAC/NBS1 in CHO cells with specific probes for the BAC vector (in red) and for gene sequences (green). (B) FISH analysis of alphoid^tetO-HAC/VHL in CHO cells. (C) Immunocytochemical staining of CHO cells and (D) isogenic cells with the alphoid^tetO-HAC/NBS1 using Abs against pNBS1 (red) and GFP (green). (E) Western blot analysis of a CHO clone containing alphoid^tetO-HAC/NBS1 with human-specific Abs against pNBS1. NBS1 inserted into the alphoid^tetO-HAC produces a protein of the predicted size.