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. 2011 Nov 28;108(50):20048–20053. doi: 10.1073/pnas.1114483108

Fig. 4.

Fig. 4.

pVHL expression in VHL-deficient cells. (A) FISH analysis of the alphoidtetO-HAC/VHL in VHL-deficient 786-0 RCC4 cells. (B) Effect of pVHL on the level of HIF2-α, CyclinD1, and Cdk1/CDC2 proteins. Immunoblots of whole cell extracts isolated from the 786-0 cell line, the same cell line containing alphoidtetO-HAC/VHL before and after induction of HAC loss. CA9 and PGK1 proteins, which are not affected by pVHL status in 786-0 cells, were used as controls. (C) The level of invasiveness of VHL-deficient 786-0 cells and the same cell line containing alphoidtetO-HAC/VHL in the presence of FBS. (D) pVHL regulation of HGF-mediated (HGF+) 786-0 cell branching morphogenesis in the absence and presence of functional pVHL (with or without alphoidtetO-HAC/VHL). (E) pVHL regulation of 786-0 cell migration. pVHL expression in 786-0 cells results in reduction of cellular migration. A clear difference was observed after 7 h when representative fields were photographed. Each experiment was performed in triplicate.