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. 2011 Nov 29;108(50):20160–20165. doi: 10.1073/pnas.1113013108

Fig. 2.

Fig. 2.

Identification of darkhelmet Tn mutants with reduced FlgRΔCTD activity in ΔflgS mutants. Arylsulfatase assay examining expression of the σ54-dependent flaB::astA transcriptional fusion in C. jejuni strains grown on MH agar. The level of flaB::astA expression in each strain is relative to WT C. jejuni producing the WT FlgSR TCS, which was set to 100 units. The FlgS and FlgR proteins produced in each strain are indicated. ΔCTD indicates FlgRΔCTD. Dashes indicate deletions of respective genes. Tn mutants are in the C. jejuni ΔastA ΔflgS flgRΔCTD flaB::astA background. Error bars indicate SDs. (Inset) Acetogenesis pathway as outlined in E. coli with minor modifications (20), and predicted to be intact in C. jejuni (24, 25).