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. 2011 Nov 28;108(50):20178–20183. doi: 10.1073/pnas.1117820108

Fig. 2.

Fig. 2.

Neurotrophin-induced CREB transcription is regulated by SUV39H1 and histone methylation. (A) PCR quantification of ChIP analysis of primary cortical neuronal cells depleted of SUV39H1 and stimulated with BDNF. CREB immunoprecipitation was followed by PCR analysis of the c-fos promoter. Densitometric analysis of CREB binding to c-fos promoter: *P < 0.01; n = 3; one-way ANOVA; mean ± SEM. (B) mRNA levels of c-fos, vaccinia nerve growth factor (vgf), and actin in primary cortical neuronal cells after depletion of SUV39H1. (C) PCR quantification of ChIP analysis of primary cortical neuronal cells transfected with wild-type SUV39H1 or SUV39H1-H324K, and stimulated with BDNF. CREB immunoprecipitation was followed by PCR analysis of the c-fos promoter. Densitometric analysis of CREB binding to c-fos promoter: *P < 0.01, n = 3, one-way ANOVA, mean ± SEM. (D) mRNA levels of c-fos and actin in primary cortical neuronal cells after transfection of SUV39H1 and SUV39H1-H324K constructs in primary cortical neurons. (E–F) Immunoblot analysis of methylated and acetylated H3K9 and actin in primary cortical neuronal cells after treatment with BDNF (E) and NGF (F). (G) Immunoblot analysis of methylated and acetylated H3K9 and actin in PC-12 cells after treatment with KCl.