Evolution of the N332A-sensitive bNAb specificity in macaque CE8J plasma. (A) Plasma samples collected at serial time points were tested for neutralizing activity against JR-CSF and MGRM-C26 pseudovirus variants containing an N332A mutation. (B) Plasma samples taken at serial time points were tested for neutralizing activity against SHIVAD8 containing an N332A mutation. Boxes are color coded as follows: gray, IC50 < 1:100; green, 1:100 < IC50 < 1:300; yellow, 1:300 < IC50 < 1:600; orange, 1:600 < IC50 < 1:1,000; red, IC50 > 1:1,000.