Fig. 1.

Nuclear size dynamics in embryonic cotyledons during seed maturation, imbibition, and germination. (A and B) Representative DAPI-stained embryonic cotyledon nuclei of Col at 10 DAP (A) and 20 DAP (B). (C) Nuclear area during seed maturation of Col (black squares) and Ler (white circles). (D) Kinetics of nuclear volume reduction during seed maturation of Ler. (E) Nuclear area during imbibition and germination of Col (black squares) and Ler (white circles). (F and G) Z-minimum projection-derived confocal microscopy images of 2 h (F) and 72 h (G) imbibed transgenic seeds expressing the nuclear marker Histone 2B fused to fluorescent GFP (Fig. S3). (H) Nuclear areas of cotyledons of 72-h imbibed Col and Ler seeds that were nongerminating (black bars) or germinating (gray bars), derived from the same seed batch. Error bars represent SE; n ≥86 (D; n ≥25), Significance levels: *0.01 > P < 0.05; ****P < 0.0001, two-tailed Student t-test compared with control. (Scale bars: 10 μm.)