Fig. 1.

Computationally predicted interaction areas for LC in β1 subunit–containing BK channels. (A) Molecular structure of LC. (B) Schematic representation of BK channels human β1 subunit with computationally predicted interaction areas for LC in TM2 domain. (C) Amino acid sequence alignment of human BK β1 TM2 and rat cerebral artery myocyte BK β1 TM2 domains. Amino acids proposed for LC interaction areas in hβ1 and rβ1 are shown in gray and colored, respectively. Snapshots from the molecular dynamics (MD) simulation for LC interactions with the BK rβ1 TM2 domain outer (D) and inner (E) regions. Hydrogen bond between TM2 Thr165 or 169 and C3-hydroxyl of LC is indicated as blue dashed line.