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Amino acid substitutions in the proposed LC sensing site on the inner region of rβ1 TM2 domain ablate LC sensitivity. Records from cbv1 + rβ1T169A (A) and cbv1 + rβ1L172A,L173A (B) show LC-insensitive currents, whereas cbv1 + rβ1L168A (C) channels retain LC sensitivity. (D) Averaged responses to LC. NS, no significant difference (P > 0.05). *P < 0.05; significant from control (vehicle).
