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Permeability of oxalate and mannitol increased in parallel with knockdown of ZO-1. Unidirectional absorptive fluxes of [14C]oxalate and [3H]mannitol were measured in control and ZO-1 knockdown cells (ZO-1 KD). Immunoblot confirming ZO-1 knockdown is shown in inset. The data are expressed as the means ± SEM (n = 4). *P < 0.05 of oxalate and mannitol permeability in ZO-1 knockdown cells versus oxalate and mannitol permeability, respectively, in control cells.
