Figure 5.

Clathrin knockdown results in aberrant Golgi reformation on mitotic exit. A) Outlines of Golgi structures thresholded and analyzed using the particle analysis algorithm (see Materials and Methods and ref. 9) in interphase (a, b) and cytokinesis (c, d). B, C) Particle analysis generated measurements for particle size (B) and particle number (C) in interphase (insets) and cytokinetic cells. All values are averages from 30 cells. D) Electron micrographs of mock- and CHC siRNA-treated NRK cells at 3 time points following release from mitotic arrest (i.e., cytokinesis): T₁ = 6 h 20 min (a, b), T₂ = 7 h (c, d), T₃ = 7 h 30 min (e, f). All images are from cytokinetic cells, as determined by cell pairs connected by a cytokinetic bridge (inset, c′). Arrowheads indicate heterogeneous clathrin structures. G, Golgi stack; N, nucleus. Scale bar = 200 nm.