Table 4. Antibodies used in the immunohistochemical study.
Fos | CTb | PHA-L | TH | ChAT | |
Primary antibodies | Rabbit | Goat | Rabbit | Rabbit | Rabbit |
Suppliers | Oncogene, USA | List Biological Labs, USA | Vector Labs, USA | Jacques Boy, France | Chemicon, USA |
Dilution | 1 :10,000 | 1 :40,000 | 1 :5,000 | 1 :5,000 | 1 :2,500 |
Secondary antibodies | Donkey anti-rabbit | Horse anti-goat | Donkey anti-rabbit | Donkey anti-rabbit | Horse anti-goat |
Suppliers | Rockland | Vector Labs | Rockland | Rockland | Vector Labs |
Dilution | 1 :1,000 | 1 :1,000 | 1 :1,000 | 1 :1,000 | 1 :1,000 |
ChAT: choline acetyltransferase, CTb: cholera toxin subunit B, PHA-L: Phaseolus vulgaris leucoagglutinin, TH: tyrosine hydroxylase.