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. 2012 Jan 3;7(1):e29383. doi: 10.1371/journal.pone.0029383

Figure 1. CXCR4 expression analysis of the cultured HSPCs.

Figure 1

(A) The expanded cells were stained using anti CXCR4 antibody and the expression was assessed by flow cytometry. HSPCs cultured in the presence of zVADfmk/zLLYfmk showed a two-fold increase in the CXCR4+ population compared to the control counterpart. Data are represented as mean percentage ± standard deviation of five biological replicates, *p≤0.05, **p≤0.01. A representative flow-overlay is shown in the inset. (B) Confocal microscopy images further confirm higher expression of CXCR4 (green) on cell surface of HSPCs from zVADfmk/zLLYfmk cultures than that of controls. Cell nuclei (blue) were stained with DAPI (bar  = 10 µm, n = 3). (C) Flow cytometry analyses of HSPCs after multicolor staining (CD34 and CXCR4 or CD34, CD38 and CXCR4) demonstrate higher CXCR4 positive population in CD34+ and CD34+CD38− compartments. Thus, zVADfmk/zLLYfmk facilitates higher production of CXCR4 expressing CD34+ and CD34+CD38− hematopoietic progenitor cells. Data are represented as mean percentage ± standard deviation of four experiments, *p≤0.5.