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. 2012 Jan 3;7(1):e29383. doi: 10.1371/journal.pone.0029383

Figure 7. In vivo homing expanded HSPCs.

Figure 7

(A) Schematic illustration of the in vivo homing assay conducted in NOD/SCID mice. (B) After 24 hours of transplantation, the control and test animals were sacrificed and the bone marrow and spleen cells were assessed for the presence of human CD45 and human CD34 markers. The animals that received the HSPCs expanded in the presence of either of the inhibitors exhibited a significantly increased migration to the bone marrow and spleen tissues. Data are represented as mean ± standard deviation of four experiments, *p≤0.05, **p<0.01. However, the CD34+ cell homing to the spleen tissues were found to be unaffected. (C & D) Blocking of CXCR4 and integrins prior to transplantation significantly reduced the bone marrow and spleen homing of expanded HSPCs compared to the unblocked respective sets. The zVADfmk/zLLYfmk HSPCs showed an increase in homing compared to control (*p≤0.05, **p<0.01, n = 3 independent experiments).