Figure 2. Detection of novel miRNA candidate identified by small RNA library (A) or degradome (B) sequencing.

Plants were grown hydroponically with full-strength Hoagland's nutrient solution for 2 weeks and then transferred to the N-free or N-replete medium for 2 days before small RNA was isolated from leaves and roots. Five micrograms of small RNA from each sample was loaded and hybridized with corresponding ³²P-labeled probes. 5s/tRNA is shown as a loading control.