Figure 5.
Coordinated ER stress response of ERAD genes. qRT-PCR results for validated and suspected ERAD components upon treatment of HEK293 cells with TUNIC (10μg/mL, 6 hr). Data are presented as fold induction (log2) normalised to β-Actin. TUNIC-induced expression changes in ERAD genes plotted as groups according to: (a) Fold induction of gene expression represented by functional category. (c) Fold induction of gene expression from panel (b) mapped onto the ERAD interactome from Fig. 2b. Additional genes of interest are presented alongside the induction map.