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. 2012 Jan 3;7(1):e29745. doi: 10.1371/journal.pone.0029745

Figure 5. Role of MMP23B in IL-11 mediated invasion of JEG-3 and HTR-8/SVneo cells.

Figure 5

Quantitative RT-PCR was done for the expression of MMP23B (Panel A) as mentioned in Materials and Methods . Each bar represents the ΔCt values after normalization with the 18S rRNA. The data is expressed as mean ± SEM of 3 experiments performed in triplicates. In another experiment, JEG-3 cells were transfected with either MMP23B siRNA or non-genomic siRNA for 72 h and end point RT-PCR was done to check the level of silencing in them, keeping 18S rRNA as internal control (Panel B). The transfected cells were used to study their invasive behavior in the presence or absence of IL-11 (200 ng/ml) as described in Materials and Methods . The results are expressed as mean ± SEM of fold change in invasion as compared to non-genomic siRNA transfected cells in the absence of IL-11, observed in 3 independent experiments. *p<0.05; **p<0.001; #p<0.01 between JEG-3 cells transfected with non-genomic and MMP23B siRNA; $p<0.001 between IL-11 treated non-genomic siRNA transfected and MMP23B siRNA transfected JEG-3 cells.