Table 2. Effect of IL-11 on the expression of MMPs, TIMPs and integrins.
| Gene | JEG-3 | HTR-8/SVneo | ||
| Control | IL-11 | Control | IL-11 | |
| MMP2 | 1±0.23 | 1.3±0.29 | 39.4±14.4# | 14.4±6.2* |
| MMP3 | 1±0.34 | 0.8±0.56 | 19.3±7.18# | 1.6±0.17* |
| MMP9 | 1±0.3 | 1.21±0.44 | 217.3±42.6# | 83.5±19.6* |
| TIMP1 | 1±0.12 | 1.4±0.59 | 139±7.00# | 111±25.00 |
| TIMP2 | 1±0.38 | 0.9±0.03 | 3.5±0.30# | 2.2±1.00 |
| TIMP3 | 1±0.39 | 0.9±0.03 | 0.9±0.07 | 0.6±0.35 |
| Integrin α5 | 1±0.45 | 1.1±0.10 | 2.9±1.55# | 2.9±1.25 |
| Integrin α6 | 1±0.24 | 0.7±0.15 | 0.9±0.05 | 0.6±0.04 |
| Integrin αV | 1±0.32 | 1.2±0.19 | 0.6±0.01 | 0.4±0.01 |
For each sample, ΔCt values were obtained after normalization with the Ct values for 18S rRNA. After that fold change in expression (ΔCt values) between the groups was calculated with respect to the untreated JEG-3 cells.
#
p<0.001 between untreated JEG-3 and HTR-8/SVneo cells;
*p<0.05 between un-stimulated and IL-11 treated HTR-8/SVneo cells.