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. 2012 Jan 3;6(1):e1443. doi: 10.1371/journal.pntd.0001443

Figure 3. Selection and screening to test ability of SmMef2 to induce reporter genes.

Figure 3

Serial dilution spot tests are displayed on –adenine or –histidine nutritional marker plates to test whether SmMef2 can induce the genes encoding adenine or histidine biosynthesis (A and B, respectively). Cells are diluted from right (undiluted) to left in 10 fold increments. Alpha galactosidase assay to test whether SmMef2 can induce the MEL1 reporter gene (C), where blue color indicates reporter gene activation. The negative control is white. To control for growth alone, cell are grown on media without tryptophan, which selects for the presence of the pGBKT7 plasmid (D).