Figure 2. Relative quantification of dectin-1 and mannose receptor transcripts in dendritic cells infected with P. brasiliensis.

BALB/c bone marrow-derived DCs were cultured for 6 h with or without P. brasiliensis yeast cells (Pb). Then total RNA was extracted from the DCs and used in qRT-PCR assays. Fold change values were determined after each gene was normalized to the constitutively expressed RPS9 gene using the comparative threshold method. Data are reported as the mean ± standard deviation. * P<0.05.