Figure 3. Effect of mannan and laminarin on the accumulation of selected immune-related transcripts in dendritic cells infected with P. brasiliensis.

Murine bone marrow-derived DCs were incubated with mannan (Man) or laminarin (Lam) at 100 and 200 µg/ml, respectively, for 30 min. Subsequently, P. brasiliensis yeast cells (Pb) were added to DCs at a ratio of 1∶1, and the co-culture was incubated for 6 h. Total RNA was extracted from DCs and used in qRT-PCR assays. Fold-change values were determined after each gene was normalized to RPS9 using the comparative threshold method. Data are reported as the mean ± standard deviation. * P<0.05 compared to the DCs+Pb/DCs group.