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. 2012 Jan 3;6(1):e1459. doi: 10.1371/journal.pntd.0001459

Table 3. Real-time PCR validation of microarray data.

Clone ID Gene Fold Change
Microarray6 h qRT-PCR*6 h
577486 CCL22 2.94 10.28±1.32
5213134 TNF-α 1.91 19.42±0.98
575033 NFκB 1.42 4.42±0.37
21961 NκRF −1.33 −1.82±0.02
6047339 TLR4 −1.26 −1.82±0.07
4753177 TLR2 NM 1.12±0.06
190887 MYD88 NM 1.06±0.06

*Fold-change values were determined after normalization to Rps9 using the comparative threshold method. Values indicate mean fold change ± SD of two independent experiments.

NM: not significantly modulated, as shown by microarray.