Table 3. Real-time PCR validation of microarray data.
Clone ID | Gene | Fold Change | |
Microarray6 h | qRT-PCR*6 h | ||
577486 | CCL22 | 2.94 | 10.28±1.32 |
5213134 | TNF-α | 1.91 | 19.42±0.98 |
575033 | NFκB | 1.42 | 4.42±0.37 |
21961 | NκRF | −1.33 | −1.82±0.02 |
6047339 | TLR4 | −1.26 | −1.82±0.07 |
4753177 | TLR2 | NM | 1.12±0.06 |
190887 | MYD88 | NM | 1.06±0.06 |
*Fold-change values were determined after normalization to Rps9 using the comparative threshold method. Values indicate mean fold change ± SD of two independent experiments.
NM: not significantly modulated, as shown by microarray.