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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Arthritis Rheum. 2012 Mar;64(3):799–807. doi: 10.1002/art.33375

Figure 2. Silencing of endogenous CREM α upregulates SYK protein expression and phosphorylation.

Figure 2

A, T cells were transfected with 500 nM control siRNA or 100 and 500 nM CREM α-specific siRNA. At 72h post-transfection cells were lysed with RIPA buffer and Western blotted with antibody against CREM α. B, Normal T cells were transfected with 500 nM of control siRNA or 100 and 500 nM CREM α-specific siRNA. Cells were collected after 72h post-transfection in RIPA buffer and Western blotted with antibody against total SYK and β-actin. Data represented as mean ± SEM of densitometry scan of 5 different blots. C, Normal T cells were transfected with 500 nM control siRNA and 100 and 500 nM CREM α-specific siRNA. 1 µg of anti-CD3/anti-CD28 stimulation was given 1h before cell harvesting. Cells were collected after 72h of post-transfection in RIPA buffer and Western blotted with antibody against phosphorylated SYK and β-actin. Data represented as mean ± SEM of densitometry scan of 5 different blots.