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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Arthritis Rheum. 2012 Mar;64(3):799–807. doi: 10.1002/art.33375

Figure 4. CREM α overexpression reduces SYK promoter activity.

Figure 4

T cells were co-transfected with 2 µg wild-type SYK promoter-luciferase reporter construct, CRE site-mutated SYK promoter-luciferase construct or 500 ng CREM α expression plasmid and 50 ng Renilla plasmid vector as internal control. DNA concentrations were normalized by empty vector. Luciferase readings from cell extract was measured and normalized with Renilla luciferase and the data was plotted as mean ± SEM based on 6 different experiments. Construct cartoon shows upstream cis elements on SYK promoter. Rectangular box indicate CRE site and AP-1 site shown as oval shape. Out of several Ets binding sites on SYK promoter a few are shown as diamond shapes, those are close to CRE and AP-1 sites.