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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mitochondrion. 2011 Sep 17;12(2):213–219. doi: 10.1016/j.mito.2011.09.001

Fig. 2.

Fig. 2

miR-181 targets 3 members of the Bcl-2 family. (a) The seed sequences of Bcl-2, Bcl-2-L11, and Mcl-1 3′UTRs targeted by miR-181 are highly conserved across species (from TargetScan). (b) Dual luciferase activity assays performed in BOSC23 cells co-transfected with the plasmid containing luciferase followed by the Bcl-2 or Bcl-2-L11 or Mcl-1 3′UTR (WT) and plasmids encoding either pri-miR-181ab or pri-miR-181cd or their seed mutants (SM) demonstrates that miR-181 recognizes all of these 3′UTRs. (c) The same assay performed with the wild type 3′UTRs of Bcl-2, Bcl-2-L11, or Mcl-1 (WT) or their seed mutants (SM) shows that miR-181ab and cd both reduce luciferase activity. Luciferase assays were performed 3 times in triplicate. *P<0.01 compared to the SM group.