Table 1.
Transgenesis efficiency of LG clones for different techniques
| Technique | Treatment | Experiments (N) |
Injected Eggs (N) |
Average Survival Rate at Hatching (% ± SD1) |
Transgenesis Efficiency (%) |
|---|---|---|---|---|---|
| phiC31 Integrase |
1ng Integrase mRNA + 20-50pg DNA |
10 | 601 | 17 ± 4 | 30 – 40 |
| non-injected | 10 | 660 | 35 ± 19 | --- | |
| SB Transposase |
0.1ng SB mRNA + 15-20pg DNA |
3 | 350 | 20 ± 13 | 12 – 35 |
| non-injected | 3 | 355 | 37 ± 12 | --- | |
| I-Sce I Meganuclease |
1x10-3 U I-Sce I + 80pg DNA |
8 | 881 | 36 ± 18 | 35 – 50 |
| non-injected | 8 | 722 | 52 ± 29 | --- | |
1
SD: standard deviation