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. 2012 Jan 4;2:209. doi: 10.1038/srep00209

Figure 4. Decay of luciferase mRNA by overexpression of CUGBP1/MBNL1.

Figure 4

(a) Schemes of luciferase reporter plasmids harboring Gapdh 3′ UTR. Each construct was made carrying either SV40 or tet-responsive promoter. (b) Luciferase activity after overexpression of CUGBP1/MBNL1. HEK293 cells were transfected with the indicated SV40-driven luciferase reporter constructs. Luciferase activity is normalized for the transfection efficiency using co-transfection of pRL/SV40. (c) Decay of luciferase mRNA after overexpression of CUGBP1/MBNL1. HEK293 Tet-off cells were transfected with the indicated tet-responsive promoter-driven luciferase reporter constructs. Doxycycline was added to the medium to stop transcription at time 0. Temporal profiles of luciferase mRNA decay were quantified by real time RT-PCR and are normalized for Gapdh mRNA levels. All experiments were triplicated, and the mean and s.d. are indicated (* p < 0.05; ** p < 0.01).