Figure 1.

Cell death was assessed by Trypan Blue assay. (A) Cells were treated 200, 400, 600, 800, 1,000 µM MnCl₂ for 24 hours and the cell viability was assessed by trypan blue assay (control cells were treated without MnCl₂). (B) Cells were treated with 1,000 µM MnCl₂ for 3, 6,12, 24, 48 hours and the cell viability was assessed by trypan blue assay. (control cells were treated without MnCl₂). (C) Cells were pretreated with 1,000 µM MnCl₂ 30minutes before followed by 1, 10, 100, 1,000 µM EDTA then incubated for 24 hours for cell viability.

Data represents mean±standard error (n = 3).

^*p < 0.05: significantly different from control cells.