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SDS-PAGE analysis of rROP2186–533. Lane M: Prestained protein ladder. Lane 1: Cell lysates from pET-28a in Escherichia coli BL21 strain. Lane 2–7: Cell lysates from pET-28a-ROP2186–533 in E. coli BL21 strain induced with isopropyl-β-D-thiogalactoside (IPTG) for 1–6 h. Lane 8: Purification products of rROP2186–533.
