Table 1.

Immunoreactive Proteins Identified by LC/MS-MS

Spot no.	Putative protein	Unique peptides	Coverage (%)	pI (Exp/Obs)a	Mr(Kda) (Exp/Obs)a	MASCOT score	Accession no.
1	Hsp70	84	63.65	5.3/5.4	73.4/72.0	830.28	BAB20284
2	Hsp70	49	47.48	5.3/5.6	73.4/72.0	480.26	BAB20284
3	Nucleoside triphosphate hydrolase 3	23	42.36	6.0/6.2	69.1/70.1	220.31	AAC80188
4	Importin β-1 subunit	2	2.59	4.4/5.2	98.3/109.1	20.27	CAJ20312
5	Hsp70	18	25.22	5.3/5.4	73.4/41.5	190.27	BAB20284
6	Hsp70	18	18.40	5.3/5.5	73.4/40.1	190.32	BAB20284
7	Actin	9	29.52	4.9/5.7	41.9/38.2	70.25	CAJ20602
8	Mitochondrial malate-dehydrogenase	4	10.43	8.9/6.5	50.3/35.2	40.36	ABU49220
9	Mitochondrial malate-dehydrogenase	13	34.68	8.9/6.8	50.3/34.1	130.24	ABU49220
10	Mitochondrial F1-ATP synthase beta subunit precursor	33	58.93	6.4/6.2	59.9/54.5	320.23	ABB17195
11	AF 123457-1 enolase	55	84.68	5.6/6.4	48.2/52.9	540.35	AAG60329
12	Hexokinase	30	41.67	5.8/6.8	51.4/53.0	310.28	BAB55664
13	AF 123457-1 enolase	14	49.32	5.6/6.9	48.2/52.1	140.24	AAG60329
14	AF 123457-1 enolase	2	7.88	5.6/7.1	48.2/50.5	20.26	AAG60329
15	Phosphoglycerate kinase 1	54	83.17	6.3/7.4	44.5/45.0	540.35	ABE76509
16	Phosphoglycerate kinase 1	4	18.57	6.3/7.8	44.5/42.1	40.38	ABE76509
17	Elongation factor 1-α	5	13.62	9.2/8.4	48.9/42.1	50.24	CAJ20335
18	Elongation factor 1-α	3	8.04	9.2/9.2	48.9/42.5	30.28	CAJ20335
19	Glucose-6-phosphate-1-dehyrogenase	17	43.57	7.4/7.7	62.7/23.5	160.36	CAJ20381
20	Serine-threonine phosophatase 2C	29	71.00	5.3/4.0	36.9/25.4	290.27	CAC86553
21	Inflammatory profilin	5	41.72	4.2/4.5	17.5/24.0	50.36	CAJ20409
C	B chain B, rop2 from Toxoplasma gondii	2	7.34	6.5/5.1	42.5/57.5	20.28	Z36906

^aExpected and observed molecular mass (Mr) and isoelectric point (pI).

LC/MS-MS, liquid chromatography/tandem mass spectrometry.